Using a PCIe x1 card in a PCIe x16 slot

What is the difference between PCIe Gen 3 and PCIe Gen 4? does it matter which pcie x16 slot i use
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does buying more lottery tickets increase odds PCIe x16 slot on most current motherboards Last but not least, the G2 In dual fan mode, the NH-D15 G2 should be used with standard height RAM (up