PCIE x16 second slot not working

PCIE x16 second slot not working pciex16 slot
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pciex16 slot configuration EZ-Latch：PCIe x16 Slot with Quick Release Design; Fast Networks： LAN & Wi-Fi 6E ; Extended Connectivity：DP, HDMI, Rear USB-C® 10Gbs, Front

ananta slot Description This PCIe x1 to PCIe x16 Slot Extension adapter provides enhanced compatibility between motherboards and a variety of PCI Express expansion cards, A slot can provide multiple lanes, from 1 lane in the smallest slot to up to 16 in the largest slots Using two graphics cards normally